Methods in Plant Molecular Biology and Biotechnology [Paperback]

_x005F_x000D_ Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods, such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.

_x005F_x000D_ The Use of Recombinant DNA Technology to Produce Genetically Modified Plants (B.M. Greenberg and B.R. Glick). Production and Analysis of Plant Mutants, Emphasizing Arabidopsis thaliana (R.L. Malmberg). DNA Sequence Organization and Gene Copy Number Determination (J.J. Pasternak). Isolation and Characterization of Plant DNAs (B.H. Taylor, J.R. Manhart, and R.M. Amasino). Isolation and Characterization of Plant mRNA (J. Strommer, R. Gregerson, and M. Vayda). Procedures for Introducing Foreign DNA into Plants (B.L. Miki, P.F. Fobert, P.J. Charest, and V.N. Iyer). Vectors for Plant Transformation (M.Y. Gruber and W.L. Crosby). Construction of l Clone Banks (J.L. Slightom, R.F. Drong, and P.P. Chee). Transient Analysis of Gene Expression in Plant Cells (A.N. Nunberg and T.L. Thomas). Techniques for Isolating and Characterizing Plant Transcription Promoters, Enhancers, and Terminators (G. An and Y. Kim). Use of Xenopus Oocytes to Monitor Plant Gene Expression (J.J. Heikkila). In Situ Localization of Specific mRNAs in Plant Tissues (H.I. McKhann and A.M. Hirsch). Immunological Methods for Assessing Protein Expression in Plants (E.B. Dumbroff and S. Gepstein). In Situ Immunocytochemical Localization of Plant Proteins (N. Benhamou and A. Asselin). Accessing Computer Software for Molecular Biology (J.J. Pasternak). Sequence Similarity Searches, Multiple Sequence Alignments and Molecular Tree Building (J.J. Pasternak). DNA Mapping in Plants (B.S. Landry). Random Amplified Polymorphic DNA (RAPD) Analysis (K.F. Yu, A. Van Deynze, and K.P. Pauls). Detection and Characterization of Plant Pathogens (P.H. Goodwin and A. Nassuth). Screening for Inoculant-Quality Strains of Rhizobia (D.H. Hubbell). Isolation and Characterization of Plant Growth-Promoting Rhizobacteria (Y. Bashan, G. Holguin, and R. Lifshitz).